Here In Turkey, I'm a Vodafone TR user and using iPhone x.
Here are my settings;
uPNP is also enabled but I don't think Wi-Fi calling is related any of these.
It's all related with your phone and operator. I can use wi-fi calling via android phone tethering over another operator which uses CGNAT. So depending on my experiences; you don't need any tricks to be done on your Asus Router.
Besides; mum has Xiaomi A1 which also supports Wi-Fi calling as far as I know but Vodafone TR does not support Wi-Fi calling for this model so mum can't use Wi-Fi calling.