For me, I was getting unbound hit rate from 30%-35% to 65%-75% once I made this change. Remove any upstream server from Cloudflare, quad9, and etcs from Adguad Home. Just include the unbound ipv4 and ipv6. For DoT, I just enabled on unbound end.
127.0.0.1:53535
tcp://127.0.0.1:53535
[::0]:53535
tcp://[::0]:53535
[/f.e.4.5.0.c.1.5.2.0.7.1.0.0.6.2.ip6.arpa/][::]:553
[/router.asus.com/][::]:553
[/www.asusnetwork.net/][::]:553
[/www.asusrouter.com/][::]:553
[/use-application-dns.net/][::]:553
[/dns.resolver.arpa/][::]:553
[/lan/][::]:553
[//][::]:553
127.0.0.1:53535
tcp://127.0.0.1:53535
[::0]:53535
tcp://[::0]:53535
[/f.e.4.5.0.c.1.5.2.0.7.1.0.0.6.2.ip6.arpa/][::]:553
[/router.asus.com/][::]:553
[/www.asusnetwork.net/][::]:553
[/www.asusrouter.com/][::]:553
[/use-application-dns.net/][::]:553
[/dns.resolver.arpa/][::]:553
[/lan/][::]:553
[//][::]:553